Journal: Molecules

Article Title: Synthesis and Biological Evaluation of Spirocyclic Chromane Derivatives as a Potential Treatment of Prostate Cancer

doi: 10.3390/molecules26113162

Figure Lengend Snippet: 22Rv1 cell antiproliferation activity on optimization of core scaffolds a .

Article Snippet: The 22Rv1 prostate cancer cell line in this study was purchased from KeyGEN Biotech Co. Ltd. (Nanjing, Jiangsu, China ).

Techniques: Activity Assay

Journal: Molecules

Article Title: Synthesis and Biological Evaluation of Spirocyclic Chromane Derivatives as a Potential Treatment of Prostate Cancer

doi: 10.3390/molecules26113162

Figure Lengend Snippet: Effect of optimization of R1, R2 and R3 on 22Rv1 cell antiproliferation activity a .

Article Snippet: The 22Rv1 prostate cancer cell line in this study was purchased from KeyGEN Biotech Co. Ltd. (Nanjing, Jiangsu, China ).

Techniques: Activity Assay

Journal: Molecules

Article Title: Synthesis and Biological Evaluation of Spirocyclic Chromane Derivatives as a Potential Treatment of Prostate Cancer

doi: 10.3390/molecules26113162

Figure Lengend Snippet: 22Rv1 cell antiproliferation and pharmacokinetic profiles of two isomers of B16 a .

Article Snippet: The 22Rv1 prostate cancer cell line in this study was purchased from KeyGEN Biotech Co. Ltd. (Nanjing, Jiangsu, China ).

Techniques: Drug discovery

Journal: Molecules

Article Title: Synthesis and Biological Evaluation of Spirocyclic Chromane Derivatives as a Potential Treatment of Prostate Cancer

doi: 10.3390/molecules26113162

Figure Lengend Snippet: In vivo 22Rv1 xenograft model study of compound B16-P2 and A-485. ( A ) The tumor growth curve of four groups. ( B ) The body weights of mice over time ( n = 6, * p < 0.05 versus control).

Article Snippet: The 22Rv1 prostate cancer cell line in this study was purchased from KeyGEN Biotech Co. Ltd. (Nanjing, Jiangsu, China ).

Techniques: In Vivo, Control

Journal: The Prostate

Article Title: SLUG is a Direct Transcriptional Repressor of PTEN Tumor Suppressor

doi: 10.1002/pros.22974

Figure Lengend Snippet: (A) Regulation of PTEN expression by SLUG in prostate cancer cells. 22RV1 (left panel) and DU145 (right panel) cells were infected with retroviruses expressing pMIGR1-Slug and pMIGR1 (control vector). Total protein was extracted from respective cell lines and analyzed by Western-blot analysis using anti-SLUG, anti-pAKT, anti-PTEN and anti-GAPDH (loading control) antibodies, respectively.

Article Snippet: RESULT We showed that overexpression of SLUG decreased expression of PTEN tumor repressor in prostate cancer cell lines 22RV1 and DU145; conversely, knockdown of SLUG expression elevated PTEN expresson at both protein and RNA level in these cells.

Techniques: Expressing, Infection, Control, Plasmid Preparation, Western Blot

Journal: The Prostate

Article Title: SLUG is a Direct Transcriptional Repressor of PTEN Tumor Suppressor

doi: 10.1002/pros.22974

Figure Lengend Snippet: (A) Western-blot analysis of PTEN expression in prostate cancer cell lines stably expressing Slug shRNA. 22RV1 (left panel) and DU145 (right panel) cells were infected with lentiviruses expressing SLUG-specific shRNAs and control shRNA, and then selected with puromycin and expanded. Total protein was extracted from respective cell lines and analyzed by Western-blot analysis using anti-SLUG, anti-pAKT, anti-PTEN and anti-GAPDH (loading control) antibodies, respectively.

Article Snippet: RESULT We showed that overexpression of SLUG decreased expression of PTEN tumor repressor in prostate cancer cell lines 22RV1 and DU145; conversely, knockdown of SLUG expression elevated PTEN expresson at both protein and RNA level in these cells.

Techniques: Western Blot, Expressing, Stable Transfection, shRNA, Infection, Control